Phosphoproteome Analysis

Icoon-Phosphoproteome-Analysis_v2Are you looking for a phosphorylated protein biomarker? Do you want to investigate downstream signaling of kinases and phosphatases in cells? NTRC is the exclusive provider of this service in Europe and Israel, which is carried out by Carna Biosciences, Inc.

RPPA characteristics

In our approach for phosphoproteome analysis, total cellular proteins are spotted on a microarray and probed with 180 different phospho-specific antibodies directed against more than 100 therapeutically relevant proteins.

Schermafbeelding 2014-02-06 om 10.50.02

Principle of reverse phase protein array (RPPA).

Click here for full list of phosphorylation sites analyzed in RPPA

Advantages

Approach

NTRC is the exclusive provider of this service in Europe and Israel, which is carried out by Carna Biosciences, Inc. Samples of cell lysates are prepared by the customer and shipped on dry ice to Carna in Japan. Upon ordering RPPA, the customer obtains a sample of lysis buffer and cell scrapers to prepare the lysates, according to the instructions provided by Carna. The Bradford reagents to measure protein concentration is also enclosed in the package.

Click here to read the instructions for preparation of cell lysate samples for RPPA

Alternatively, NTRC can prepare RPPA samples for Oncolines™ and SynergyFinder™ customers. RPPA is a complementary technique to study effect seen in Oncolines™ cancer cell line profiling, or SynergyFinder™ experiments at a molecular level.

In short, the work flow is as follows (for clients in Europe and Israel only):

Literature
Masuda, M et al. Alternative Mammalian Target of Rapamycin (mTOR) Signal Activation in Sorafenib-resistant Hepatocellular Carcinoma Cells Revealed by Array-based Pathway Profiling (2014) Mol. Cell. Proteomics; 13, 1429-1438.